温控表达载体的构建及HIV-1p24的表达与纯化

通过改造原核表达载体ｐ Ｂ Ｖ２２０ 和ｐ Ｅ Ｔ２８,构建出了一种新的通用型温控原核表达载体ｐ Ｖ Ｖ５,该载体携带 Ｐｒ Ｐｌ串联温控启动子以及 Ｈｉｓ Ｔａｇ 的纯化标签,利于目的蛋白的表达与纯化将 Ｈ Ｉ Ｖ１ ｇａｇ 基因的１１４８１８５７ 编码序列分别插入到ｐ Ｖ Ｖ５ｂ、ｐ Ｅ Ｔ２８ｂ 的相应位点,构建了重组表达质粒 ｐ Ｅ Ｇ１ｂ、ｐ Ｂ Ｇ７ｂ,二者在不同受体菌中,表达重组蛋白的量分别占全菌体蛋白总量的 ４２％ 和２８％ 表达产物为融合蛋白并主要以包涵体的形式存在 该蛋白 Ｎ 端与含６ 个组氨酸在内的３０ 个氨基酸残基的多肽相连,利用 Ｉ Ｍ Ａ Ｃ金属螯和层析柱,对包涵体中的重组ｐ２４ 蛋白进行纯化, 其纯度超过 ８０％ ;对上清中的可溶性ｐ２４ 蛋白进行纯化,产物最终纯度超过６７％ 纯化后的重组蛋白可与 Ｈ Ｉ Ｖ１ 型标准阳性血清发生较强的免疫学反应     

腺病毒载体与肿瘤基因治疗

基因导入系统是恶性肿瘤基因治疗中急需解决的问题．由于腺病毒作为载体介导外源效应基因在肿瘤细胞中的高效表达,使其应用日趋增多．用于肿瘤基因治疗的腺病毒可分为复制缺陷型和复制型．常用的效应基因包括：抑癌基因,前药转换酶基因,核酶基因和细胞因子基因等     

Invertibility of linear differential operators with closed range and poisson coefficients

The invertibility and injectivity properties of linear differential operators with closed range and Poisson coefficients are studied in the context of their equivalence in several spaces of vector functions defined on the axis. Translated fromMatematicheskie Zametki, Vol. 65, No. 1, pp. 143–147, January, 1999.     

Constant Mean Curvature Surfaces Foliated by Circles in Lorentz–Minkowski Space

We prove that a spacelike surface in L3 with nonzero constant mean curvature and foliated by pieces of circles in spacelike planes is a surface of revolution. When the planes containing the circles are timelike or null, examples of nonrotational constant mean curvature surfaces constructed by circles are presented. Finally, we prove that a nonzero constant mean curvature spacelike surface foliated by pieces of circles in parallel planes is a surface of revolution.     

Ample and Spanned Rank-2 Vector Bundles with k-Jet Ample Determinant on Algebraic Surfaces

We investigate ample and spanned rank-2 vector bundles E with k-jet ample determinant on algebraic surfaces S. In paticular, we classify such polarized pairs (S,E) with small second Chern class. The case of c_2(E)=k+1 is a generalization of the result of Ballico and Lanteri.     

Three- and Four-Dimensional Einstein-like Manifolds and Homogeneity

The aim of this paper is to study three- and four-dimensional Einstein-like Riemannian manifolds which are Ricci-curvature homogeneous, that is, have constant Ricci eigenvalues. In the three-dimensional case, we present the complete classification of these spaces while, in the four-dimensional case, this classification is obtained in the special case where the manifold is locally homogeneous. We also present explicit examples of four-dimensional locally homogeneous Riemannian manifolds whose Ricci tensor is cyclic-parallel (that is, are of type A) and has distinct eigenvalues. These examples are invalidating an expectation stated by F. Podestá and A. Spiro, and illustrating a striking contrast with the three-dimensional case (where this situation cannot occur). Finally, we also investigate the relation between three- and four-dimensional Einstein-like manifolds of type A and D'Atri spaces, that is, Riemannian manifolds whose geodesic symmetries are volume-preserving (up to sign).     

Yamabe不变量与在非紧流形上预定纯量曲率

我们得到关于Ｙａｍａｂｅ不变量的某些条件,使得非紧流形通过共形形变后,纯量曲率为常数．     

向量变分和向量拟变分不等式解的存在性定理及应用

本文利用纯量化方法及ＫＫＭ技巧,研究向量变分和向量拟变分不等式解的存在性问题,作为应用本文还研究了向量互补问题及最优化问题解的存在性及其等价性刻划。本文结果改进和推广了已有的结果。     

GLOBAL TOPOLOGICAL PROPERTIES OF HOMOGENEOUS VECTOR FIELDS IN R^3

In this paper the authors prove that the flows of homogeneous vector field     

ON SYMMETRIC SCALAR CURVATURE ON

1.IntroductionGivenacontinuousfunctionRonthestandardsphereS',itisaninterestingproblemwhetherRcanbethescalarcurvatureofsomemetricgwhichispointwiseconformaltothestandardmetricgoonS2.Ifwesetg~e"go,whereuisafunctiononS',theproblemisequivalenttothesolvabilityofthefollowingPDE:--A..u 2--Re"=0,onS2.(l'1)KazdanandWarner[9]pointedoutthatitmaybeinsolvable.Inthelastfewyears,alotofworkhasbeendonetosolveproblem(l.l),especiallywhenRpossessessomekindsofsymmetries.AfterthepioneerworkduetoMoser[lo]forthe…